Journal: Scientific Reports

Article Title: Identification of Two Mannosyltransferases Contributing to Biosynthesis of the Fungal-type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus

doi: 10.1038/s41598-018-35059-2

Figure Lengend Snippet: Analyses of OMGM length in ∆ glfA , ∆ glfA ∆ cmsA and ∆ glfA ∆ cmsB strains. OMGMs were prepared from ∆glfA, ∆glfA∆cmsA , and ∆glfA∆cmsB strains and analyzed by gel filtration chromatography using a Bio-Gel P-2 (2 × 90 cm) column and dH 2 O as eluent. Arrows indicate elution times of void (Vo), mannose (M1), and mannobiose (M2). The sugar eluted was detected by the phenol-sulfuric acid method. The acid hydrolysis product of dextran was used as a molecular weight marker.

Article Snippet: We extracted total GMs from ∆ glfA , ∆ glfA ∆ cmsA , and ∆ glfA ∆ cmsB strains, released OMGMs by β-elimination treatment, and evaluated the lengths of these released OMGMs by gel filtration chromatography (Bio-Gel P-2 column, 2 × 90 cm, Bio-rad) (Fig. ) with detection using the phenol-sulfuric acid method.

Techniques: Filtration, Chromatography, Molecular Weight, Marker